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Database error: Invalid SQL: select * from pwn_comment where pid='321047' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='321047' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\fcxjsm\includes\db.inc.php:73] #1 dbbase_sql->query(select * from {P}_comment where pid='321047' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\fcxjsm\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\fcxjsm\includes\common.inc.php:518] #3 printpage() called at [D:\wwwroot\fcxjsm\comment\html\index.php:13]
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发布于:2019-6-4 04:06:31  访问:13 次 回复: 篇
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Decreased GFAP positive cells compared to WT, with Cav-3 KO cells
Bottom left, WB analysis of GAPDH in whole cell lysates (WCL) from which SDF get DM-3189 pubmed ID:https://www.ncbi.nlm.nih.gov/pubmed/26104484 were generated for each group. To confirm these findings, age-matched hippocampi were examined by IF for Iba1 (microglia), GFAP (astrocytes) (Figure 4C) and MAP2 (neuronal dendrites) (Figure 4D). Cav-1 KO brains exhibit slightly increased Iba1 positive microglia and GFAP positive staining in CA1 and dentate gyrus (DG) compared to WT, similar to previously reported findings from our group [19]. Hippocampi from Cav-3 KO brainsNiesman et al. Journal of Neuroinflammation 2014, 11:39 http://www.jneuroinflammation.com/content/11/1/Page 8 ofWT, Cav-1 and Cav-3 KO were analyzed by WB for Iba1, GFAP and neuronal specific 3-tubulin to assess the cellspecific protein expression pattern (Figure 4F). Iba1 was significantly reduced (P < 0.001) in Cav-1 KO and significantly elevated in Cav-3 KO (P = 0.04). GFAP was significantly reduced (P = 0.01) and 3-tubulin was elevated (P = 0.004) in Cav-3 KO, findings consistent with IF data.Caveolin-1 knock-out and Caveolin-3 knock-out mice exhibit larger lesion volume 24 hours post-controlled cortical impact compared to wild-type miceTo assess brain injury after CCI, serial coronal sections of the brain were prepared and stained with Masson‘s trichrome and lesion volume was quantitated as previously described [22]. Both Cav-1 (n = 4; 11.9 ?1.2 mm3) and Cav-3 KO (n = 4; 15.1 ?2.2 mm3) had a significantly larger lesion volume compared WT (n = 4; 7.5 ?0.8 mm3) and sham (n = 4; 0.8 ?0.4 mm3) (P < 0.0001, Figure 5) 24 hours post-CCI.Controlled cortical impact enhances pro-inflammatory cytokines and chemokines in caveolin-1 knock-out and caveolin-3 knock-out mice at 4 hours post-impactFigure 3 Caveolin (Cav)-1 knock-out (KO) and Cav-3 KO mice have altered expression and membrane/lipid raft (MLR) localization of key neuronal and glial proteins. Sucrose density fractionation (SDF) and Western blot (WB) of wild-type (WT), Cav-1 KO, and Cav-3 KO brains. Buoyant fractions (BF) contain the cholesterol and sphingolipid enriched MLR, while heavy farctions (HF) contain non-MLR cellular components. (A) WB detection of PSD-95, NR2B, NR1 and TrkB expression in BF and HF. (B) WB detection of toll-like receptor-4 (TLR4), AA2AR, A3AR, and A1AR expression in BF and HF. (C) WB detection of LRP-1 and LDL-R expression in BF and HF. Bottom left, WB analysis of GAPDH in whole cell lysates (WCL) from which SDF PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/26104484 were generated for each group. Bottom right, WB shows loss of Cav-1 and Cav-3 protein expression in the transgenic mouse used in the present study.displayed less GFAP positive cells in the CA1 and DG compared to both WT and Cav-1 KO. WT MAP2 labeling of CA1 pyramidal neurons shows a typical pattern of normally arranged neuronal cell layer and aligned processes in the molecular layer of the DG. Cav-1 KO showed less MAP2 positive neurites, which is consistent with our previous findings [19], yet Cav-3 KO exhibited greater MAP2 staining compared to Cav-1 KO and WT (Figure 4D).
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