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Database error: Invalid SQL: select count(id) from pwn_comment where pid='313108' and iffb='1'
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select count(id) from pwn_comment where pid='313108' and iffb='1') called at [D:\wwwroot\fcxjsm\includes\db.inc.php:73] #1 dbbase_sql->query(select count(id) from {P}_comment where pid='313108' and iffb='1') called at [D:\wwwroot\fcxjsm\comment\module\CommentContent.php:65] #2 CommentContent() called at [D:\wwwroot\fcxjsm\includes\common.inc.php:518] #3 printpage() called at [D:\wwwroot\fcxjsm\comment\html\index.php:13]
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Warning: mysql_query() [function.mysql-query]: Unable to save result set in D:\wwwroot\fcxjsm\includes\db.inc.php on line 67
Database error: Invalid SQL: select * from pwn_comment where pid='313108' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='313108' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\fcxjsm\includes\db.inc.php:73] #1 dbbase_sql->query(select * from {P}_comment where pid='313108' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\fcxjsm\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\fcxjsm\includes\common.inc.php:518] #3 printpage() called at [D:\wwwroot\fcxjsm\comment\html\index.php:13]
Warning: mysql_fetch_array(): supplied argument is not a valid MySQL result resource in D:\wwwroot\fcxjsm\includes\db.inc.php on line 80
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发布于:2019-5-27 17:56:30  访问:36 次 回复: 篇
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2L3), E3 (parkin), and Ub-biotin, two) in the absence from the E
3-MethylindoleSolvent spiralis E2, TsUBE2L3, had been identified in the boxed section in the gel. 50 g of SP have been de-glycosylated by PNGase therapy and analyzed by LC/MS/MS (10 g of glycosylated proteins have been analyzed for comparison). Peptides matching a T. spiralis E2, TsUBE2L3, had been identified in the boxed section in the gel. doi:ten.1371/journal.ppat.1005977.gthe T. spiralis UniProt proteome, its reverse complement, frequent contaminants of SDS-PAGE and mass spectrometry as well as the rat UniProt proteome. Significantly less than 1 in the protein matches have been rat proteins. To validate the information, the experiment was repeated. Peptides from both the initial and second experiment have been combined and assembled into proteins. The information was cross-referenced having a prior study in the T. spiralis secretome by Robinson et al. identifying each of the same proteins, plus numerous new ones[17?9]. Proteins previously identified in T. spiralis SP applying alternative procedures were also present amongst the results[20?5]). To prevent contamination with proteins released from dead or dying parasites, uncoiled and floating T. spiralis larvae have been removed before culturing. Furthermore, to ensure a low amount of parasite death, larvae have been cultured for 24 h only, before supernatant was collected for SP purification. One particular Ub enzyme match was made to a putative protein: `ubiquitin-conjugating enzyme E2 L3 (fragment)‘. This sequence is annotated as an incomplete open reading frame (ORF) for a UBE2L3 ortholog (UniProt E5S8T6/GI:339240047/ T.sp_00154). UBE2L3, also known as UbcH7, E2-F1, UbcM4, L-UBC and UBCE7, is actually a UBCc domain Ub conjugating enzyme [26,27]. The annotated fragment ORF for TsUBE2L3 inside the database is 145 amino acids in length and lacks a start out codon. The UBCc domain of the putative TsUBE2L3 spans the fragment from the initial amino acid for the 140th amino acid. Peptides matching TsUBE2L3 PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/22928863 had been located in PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/19808328 the segment from the gel annotated in Fig 1B, corresponding to its predicted size of 17kDa. TsUBE2L3 was predicted to possess no signal peptide (signalP, iPSORT), and to be situated mainly within the cytoplasm with trace amounts in peroxisomes, nucleus and extracellular space (WolfPSORT scores of 23, three, two and two, respectively)[28?0]. All protein matches labeled as `uncharacterized‘ have been additional analyzed employing BLAST and Wise, and no other Ub enzyme domains had been identified.PLOS Pathogens | DOI:10.1371/journal.ppat.1005977 November 21,four /Secretion of a Ub E2 Enzyme by a Parasitic WormTsUBE2L3 is responsible for the Ub conjugation activity in T. spiralis SPThe putative T. spiralis UBE2L3 sequence includes 62.three of your very same residues as the human UBE2L3. A commercially readily available antibody raised against a region of the human enzyme with higher identity for the T. spiralis protein was used to analyze T. spiralis SP by immuno-blot for the presence of UBE2L3. Lysate of T. spiralis muscle larvae and human HEK 293T cells have been also analyzed as constructive controls (Fig 2A). In HEK 293T lysate one prominent band was observed, corresponding to the predicted size of your human UBE2L3 isoform 1, which can be 17.9 kDa in size[31]. Inside the lysate of T.
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