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MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select count(id) from pwn_comment where pid='291372' and iffb='1') called at [D:\wwwroot\fcxjsm\includes\db.inc.php:73] #1 dbbase_sql->query(select count(id) from {P}_comment where pid='291372' and iffb='1') called at [D:\wwwroot\fcxjsm\comment\module\CommentContent.php:65] #2 CommentContent() called at [D:\wwwroot\fcxjsm\includes\common.inc.php:518] #3 printpage() called at [D:\wwwroot\fcxjsm\comment\html\index.php:13]
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Warning: mysql_query() [function.mysql-query]: Unable to save result set in D:\wwwroot\fcxjsm\includes\db.inc.php on line 67
Database error: Invalid SQL: select * from pwn_comment where pid='291372' and iffb='1' order by id limit 0,10
MySQL Error: 1194 (Table 'pwn_comment' is marked as crashed and should be repaired)
#0 dbbase_sql->halt(Invalid SQL: select * from pwn_comment where pid='291372' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\fcxjsm\includes\db.inc.php:73] #1 dbbase_sql->query(select * from {P}_comment where pid='291372' and iffb='1' order by id limit 0,10) called at [D:\wwwroot\fcxjsm\comment\module\CommentContent.php:167] #2 CommentContent() called at [D:\wwwroot\fcxjsm\includes\common.inc.php:518] #3 printpage() called at [D:\wwwroot\fcxjsm\comment\html\index.php:13]
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发布于:2019-5-10 09:45:22  访问:45 次 回复: 篇
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Anelles, suggesting that DEK1 localizes to endosomal compartments. DEK1-CALP Failed
As an alternative, plants that segregated for DEK1/dek1-1 as well as the transgene carried only 15 of standard collapsed dek1-1 mutant seeds (Figure 3). Upon closer inspection, the ten deficit of commonly collapsed seeds could possibly be accounted for by a novel class of intermediate-type defective seeds that failed to turn green but didn‘t completely collapse when the seeds matured (Figure 3A). Comparable to typicaldek1-1 endosperm, the intermediate seed form failed to differentiate an aleurone layer (Figure 3F), which is clearly observable inside the late developmental MDL 72222Description stages of wild-type seeds (Figure 3E). In all crosses, we observed a three:1 ratio of wild-type to standard dek1 endosperms lacking aleurone cells. These experiments showed that Zm DEK1-CALP alone is incapable of rescuing the dek1 mutant maize endosperm phenotype when expressed under the control on the 27-kD g-Zein promoter. Subsequent, we expressed At DEK1-CALP in heterozygous DEK1/ dek1-1 Arabidopsis plants below the manage in the At DEK1 promoter that we had previously applied to complement the dek1-1 mutant phenotype (Lid et al., 2005). Self-pollinated DEK1/dek1-1 plants are anticipated to carry PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/27463369 a ratio of 3:1 wild-type to collapsedFigure 2. Colocalization of DEK1 and FM4-64 in Endocytotic Compartments. (A) to (C) Confocal microscopy pictures of maize root cells stained with FM4-64 (A) and with all the DEK1 Zm1 antibody in addition to a secondary antibody conjugated with FITC (B). (C) shows a merged image of (A) and (B). Yellow indicates overlapping fluorescent signal (arrowheads). (D) to (F) Confocal microscopy photos of root cells stained using the endocytic marker FM4-64 (D) and DEK1 Zm8 antibody having a FITC-conjugated secondary antibody (E). (F) shows a merged image of (D) and (E). Yellow indicates overlapping fluorescent signal (arrowheads). Note the colocalization involving DEK1 antibodies and FM4-64 in punctate cytoplasmic structures, most likely endosomes (arrowheads) Bars ?five mm.DEK1 in Maize and Arabidopsisdek1-1 seeds (Lid et al., 2005). As an alternative, plants that segregated for DEK1/dek1-1 also as the transgene carried only 15 of typical collapsed dek1-1 mutant seeds (Figure 3). Upon closer inspection, the 10 deficit of usually collapsed seeds could be accounted for by a novel class of intermediate-type defective seeds that failed to turn green but didn‘t fully collapse when the seeds matured (Figure 3A). Microscopy examination revealed that, in contrast with common dek1-1 seeds with underdeveloped endosperm and embryo, the intermediate seeds created a bigger endosperm that was retained longer than normal (Figures 3B to 3D). In wild-type seeds, the whole endosperm, using the exception of the aleurone layer, became degraded because the embryo reached maturity (Figures 3B and 3E). Related to typicaldek1-1 endosperm, the intermediate seed variety failed to differentiate an aleurone layer (Figure 3F), that is clearly observable in the late developmental stages of wild-type seeds (Figure 3E). The embryos of the intermediate seeds had been typically larger and contained a lot more cells (Figure 3G) than the homozygous dek1-1 embryos (Figure 3H). Equivalent to dek1-1 knockout embryos, embryos of the intermediate seed kind lacked proper organ formation.
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